Fig. 3

Function of CaGDT1 and CaPMR1 in calcium uptake, calcineurin signaling and gene expression. a Ca²⁺ accumulation in C. albicans cells lacking CaGDT1 and/or CaPMR1. Cells of the wild type (CAI4), the gdt1/gdt1 mutant, the pmr1/pmr1 and the gdt1/gdt1 pmr1/pmr1 mutant were grown to log-phase in YPD medium in the presence of ⁴⁵Ca²⁺, and OD_600nm values and intracellular ⁴⁵Ca²⁺ levels of these strains were measured. b β-galactosidase activities of UTR2-lacZ reporter in the wild type and the three mutant cells in the absence or presence of 300 mM CaCl₂. *indicates the statistically significant differences between each mutant and the wild-type (p < 0.05). ^#indicates the significant difference between the gdt1/gdt1 pmr1/pmr1 mutant and each of its two single-gene deletion mutants (< 0.05). c Shared differentially expressed genes (DEGs) between cells lacking the gdt1/gdt1, the pmr1/pmr1 or the gdt1/gdt1 pmr1/pmr1 mutants. DEGs for each mutant are obtained from its comparison to the wild type, which include 249 (=116 + 29 + 76 + 28) genes for the gdt1/gdt1, 446 (=197 + 29 + 76 + 144) genes for the pmr1/pmr1 mutant, and 440 (=192 + 28 + 76 + 144) genes for the gdt1/gdt1 pmr1/pmr1 mutant, respectively. Total numbers of shared DEGs between two, or among three, mutants are indicated