Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The exon 19-deleted EGFR undergoes ubiquitylation-mediated endocytic degradation via dynamin activity-dependent and -independent mechanisms

Fig. 5

The endocytic degradation of the exon 19-deleted EGFR is independent of RTN3. a, HCC827 cells stably transfected with the pGIPZ control vector and RTN3 shRNA expressing vectors (sh1–4) were lysed. Cell lysates were subjected to immunoblotting analyses with indicated antibodies. The column chart below shows the quantification data of EGFR expression. b, HCC827 parental cells and HCC827 cells stably transfected with the pGIPZ control vector, RTN3 shRNA 3 and RTN3 shRNA 4 vectors were stimulated with EGF at 100 ng/ml for indicated times. Cell lysates were analyzed by immunoblottings with indicated antibodies. c, quantification data of EGFR levels in b. d, H1650 cells stably transfected with the pGIPZ control vector, RTN3 sh3 and sh4 expressing vectors were incubated with EGF at 100 ng/ml for indicated times. Cell lysates were analyzed by immunoblottings with indicated antibodies. e, quantification data of EGFR levels at indicated time points from d. All error bars represent the standard error of the mean (n = 3)

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X