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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The exon 19-deleted EGFR undergoes ubiquitylation-mediated endocytic degradation via dynamin activity-dependent and -independent mechanisms

Fig. 4

Stress-induced endocytic degradation of mutant EGFR. a, control and dynasore-pretreated H1650 cells were incubated with EGF at 1.5 and 20 ng/ml for 4 h, and EGFR was detected by immunoblottings. The column chart below shows the quantification data of EGFR. b, control and dynasore-pretreated HCC827 cells were incubated with 100 ng/ml of EGF for 2 and 4 h or left untreated (0 h). Cell lysates were analyzed by immunoblottings with indicated antibodies. The column chart below shows the quantification data of EGFR. c, HCC827 was treated with the HSP90 inhibitor 17-AAG for indicated times and lysed. EGFR levels were determined using immunoblottings. d, immunofluorescence analysis of HCC827 treated with 17-AAG for indicated times. Images were captured using a fluorescent microscope (Olympus BX63, 40X objective). e, HCC827 cells were treated with 17-AAG (500 nM), filipin (1 μg/ml), dynasore (80 μM), dyngo-4a (20 μM), or various combinations as indicated. Cellular EGFR levels were examined by immunoblottings. All error bars represent the standard error of the mean (n = 3), and * indicates p < 0.05

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