Fig. 1
Low expression of miR-644a in HCC tissues negatively correlates with HSF1 expression and a poor prognosis. (a) Representative images (× 400) show in situ hybridization (ISH) of miR-644a in HCC and adjacent peri-cancerous liver tissues. (b) Quantitative analysis of miR-644a expression in HCC and adjacent peri-cancerous liver tissues (n = 135/group) based on ISH. Note: *** denotes P < 0.001 when compared to adjacent tissues. (c) Representative IHC images (× 400) show HSF1 expression in HCC and adjacent peri-cancerous tissues (n = 135/group). (d) Quantitative analysis of HSF1 expression in HCC and adjacent peri-cancerous liver tissues (n = 135/group) based on IHC. Note: *** denotes P < 0.001 when compared to adjacent tissues. (e) Quantitative RT-PCR analysis of miR-644a expression in HCC and adjacent peri-cancerous liver tissues (n = 135/group). Note: *** denotes P < 0.001 when compared to adjacent tissues. (f) Spearman’s test results show low miR-644a expression that negatively correlates with high HSF1 expression in HCC tissues (R2 = 0.304, P < 0.05). (g-h) Kaplan-Meier survival analysis demonstrates a correlation between miR-644a expression in HCC patients and their overall survival (g) and recurrence-free survival (h)