Fig. 3
Knockdown of SOD1 suppresses NPC cell growth in vivo. a 5-8F and CNE2 cells were infected with Ad-shCon or Ad-shSOD1 adenovirus. At 72 h after infection, cell lysates were immunoblotted for SOD1. b The 5-8F and CNE2 cells were subcutaneously injected into the left (Ad-shCon, 1 × 106/mouse) and right flank (Ad-shSOD1, 1 × 106/mouse) of nude mice. Representative images of tumours derived from the indicated groups are shown. c The weight of the mice in the indicated groups was evaluated at each time point. d and e The tumour growth curves (left panel) and tumour weights (right panel) of the indicated group of mice were measured and recorded. f Paraffin-embedded tumour sections from mice with CNE2 and5-8F cell xenografts were stained with haematoxylin and eosin or SOD1 antibody. Apoptotic cells were visualized and quantified by TUNEL staining (green) and were counterstained with DAPI (blue).Scale bar: 100 μm. All error bars represent the S.D. of results from five mice.*p < 0.05, **p < 0.01, versus the corresponding control