Fig. 7
Inhibition of αIIbβ3 attenuates NET-induced platelet response. a Representative aggregation traces showing the effect of Reopro (25 μg/mL) and RGDS (100 μM) on NET-induced platelet aggregation. WP (3 × 108/mL) were pre-incubated with the inhibitors for 15 min at 37 °C before addition of NETs. Both Reopro and RGDS reduced platelet aggregation. Platelet aggregation was measured by light transmission aggregometry (Chrono-log). b Bar graph comparing the effect of Reopro (25 μg/mL) and RGDS (100 μM) on NET-induced platelet aggregation. Platelet aggregation percentage was calculated after 20 min on a light transmission aggregometer. Results were normalised for each donor relative to NET-induced platelet aggregation. Data are expressed as mean ± SEM,***P < .001. c-f Bar graphs comparing the effect of Reopro (25 μg/mL) and RGDS (100 μM) on platelet activation (measured by P-selectin and active αIIbβ3 expression, n = 3) and ATP/ADP secretion (n = 5) elicited by NETs. In all assays, NETs constituted 10% of final reaction volume and contains 292 ± 172 pg/mL of NET-elastase. Results were normalised for each donor relative to NET-induced platelet response. Data are expressed as mean ± SEM, *P < 0.05; **P < 0.01, ns: non-significant