Fig. 5
The effects of downregulation and upregulation of AnnexinA7 on the expression levels of SDF1/CXCR4 in hepatocarcinoma cells in vitro and in vivo. Transcriptome sequencing heat maps (A1, A2) and cDNA expression (A3) of SDF1/CXCR4 in FA7DOWN/FSHUS and PA7UP/PNCEV cells. qRT-PCR (B1, B2) and Western blot (C1, C2) analysis of SDF1/CXCR4 mRNA and protein expression respectively, in FA7DOWN, FSHUS, PA7UP, and PNCEV cells in vitro (Left) and in vivo (Right). mRNA expression in FA7DOWN and FSHUS cells (B1, Left) as well as in PA7UP/PNCEV cells (B2, Left) in vitro. mRNA expressions in FA7DOWN and FSHUS cells (B1, Right) as well as in PA7UP/PNCEV cells (B2, right) in vivo. Protein expressions in FA7DOWN and FSHUS cells (C1, Left) as well as in PA7UP, PNCEV cells (C2, Left) in vitro. Protein expressions in FA7DOWN and FSHUS cells (C1, Right) as well as in PA7UP and PNCEV cells (C2, Right) in vivo. mRNA results between different groups in vitro and in vivo (E). Western blot OD densities of SDF1/CXCR4 in FSHUS and FA7DOWN cells (D1, Left) as well as in PA7UP and PNCEV cells (D2, Left) in vitro. Western blot OD densities of SDF1/CXCR4 in FSHUS and FA7DOWN cells (D1, Right) as well as in PA7UP and PNCEV cells (D2, Right) in vivo