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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling

Fig. 3

Ewing sarcoma cell lines group into CXCR4-high and -low surface expression. a Western blot analysis of total CXCR4 protein reveals multiple isoforms. Cells were grown to 70–80% confluence in standard conditions. Actin served as loading control. (b)-(d) CXCR4 surface expression distinguishes CXCR4-high and -low cell lines. Cells were grown as to 70–80% confluence in standard conditions and analyzed for CXCR4 surface expression by flow cytometry. LAN-5 neuroblastoma cells served as CXCR4-high positive control. b Representative flow cytometry plots; open graphs represent isotype-antibody controls. c CXCR4 positive cell populations depicted as mean ± SD of three independent analyses. d Relative fluorescence intensities of CXCR4 positive cell populations in (c). Corresponding data in serum-free conditions are provided in Additional file 1

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Cell Communication and Signaling

ISSN: 1478-811X