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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling

Fig. 1

Plerixafor (AMD3100) but not CXCL12 promotes proliferation of Ewing sarcoma cell lines in vitro. a Plerixafor induces a dose-dependent increase in relative cell number. Cells were cultured in standard growth conditions (10% serum) and treated as indicated for 72 h before analysis of relative cell viability and proliferation by WST-1 colorimetric assay. Statistical significance was calculated for pairwise comparisons of relative cell number at 10 μM of plerixafor and respective non-treated control. b CXCL12 (SDF-1α) ligand alone does not significantly affect relative cell numbers. c CXCL12 (100 ng/ml) does not alter proliferative effects of plerixafor (10 μM). Assays were performed as in (a). Graphs (a-c) represent the mean ± standard deviation (SD) of at least three independent experiments. d Plerixafor-associated increase in relative cell numbers is not due to apoptosis of non-treated control cells. Cells grown in standard conditions were synchronized and treated for 72 h before analysis of DNA content by propidium iodine flow cytometry. Graphs represent the mean of three experiments

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