Fig. 1From: The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signalingPlerixafor (AMD3100) but not CXCL12 promotes proliferation of Ewing sarcoma cell lines in vitro. a Plerixafor induces a dose-dependent increase in relative cell number. Cells were cultured in standard growth conditions (10% serum) and treated as indicated for 72 h before analysis of relative cell viability and proliferation by WST-1 colorimetric assay. Statistical significance was calculated for pairwise comparisons of relative cell number at 10 μM of plerixafor and respective non-treated control. b CXCL12 (SDF-1α) ligand alone does not significantly affect relative cell numbers. c CXCL12 (100 ng/ml) does not alter proliferative effects of plerixafor (10 μM). Assays were performed as in (a). Graphs (a-c) represent the mean ± standard deviation (SD) of at least three independent experiments. d Plerixafor-associated increase in relative cell numbers is not due to apoptosis of non-treated control cells. Cells grown in standard conditions were synchronized and treated for 72 h before analysis of DNA content by propidium iodine flow cytometry. Graphs represent the mean of three experimentsBack to article page