Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6

Fig. 5

HDAC8 might be primary tubulin deacetylase in HeLa cells. a Immunoblot showing the protein expression levels of HDAC8 and HDAC6 in various cancer cell lines. b Densitometric analysis of the (a) HDAC6 bands and (b) HDAC8 bands of immuoblot A. c Real time analysis confirms higher HDAC8 expression compared to HDAC6 in HeLa and HEK 293T normalized with GAPDH * indicates p-value <0.0001. d Protein expression pattern of HDAC8 & HDAC6 in HeLa and HEK 293T. e Densitometric analysis of bands in Immunoblot D. f HDAC enzyme activity assay performed with IP Anti-HDAC8 & IP Anti-HDAC6 with HeLa &HEK 293T lysates respectively. * indicates p-value <0.0001. g Increased ac-alpha tubulin levels in presence of PCI-34051 (20 μM) when compared to tubastatin μM treated condition in HeLa, whereas, tubastatin (5 μM) treatment in HEK 293T increases ac-alpha tubulin levels compared to PCI-34051 (20 μM) treated condition. h Densitometry graphs of the immunobands in G. i Real time PCR analysis of the mRNA expression of HDAC8 in HEK 293T and HeLa upon siRNA transfection. j Immunoblot analysis of HDAC8 and ac-Alpha tubulin in HDAC8 knockdown HeLa (a) and HEK 293T (b) cells. k IP-Co-IP studies of HDAC8 & HDAC6 in HeLa and HEK 293T

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X