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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6

Fig. 4

a HDAC8 interacts and deacetylates alpha tubulin. a HDAC8 and Acetylated Alpha tubulin Interaction in HeLa and HEK 293 T cells as demonstrated by immunoprecipitation using HDAC8 antibody followed by immunoblot analysis using ac-alpha tubulin. b ac-alpha tubulin levels increase when HDAC8 is inhibited with PCI-34051 (20 μM) or when treated with PKA activator, Forskolin (10 μM) and in HeLa and HEK 293 T cells. c Densitometry analysis of the immunoblot bands showing fold change in expression pattern of HDAC8, Alpha tubulin and acetylated tubulin normalized with GAPDH. d Lower panel of chromatogram represents for Unac-alpha tubulin peptide with a retention time of 14.70 Min. Middle panel of chromatogram for ac-alpha tubulin with retention time of 15.37 Min. Upper panel of chromatogram for GST-HDAC8 (30 μg) + ac-alpha tubulin (500 nM) with a retention time of 14.72 Min. Shift in the retention time from a (15.37) to b (14.72) clearly demonstrates the in vitro deacetylation of ac-alpha tubulin by GST-HDAC8

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