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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6

Fig. 2

HDAC8 co-precipitates with Alpha tubulin. a Coommassie stained gel with input and HDAC8-immunoprecipitated HeLa cell lysate along with protein marker. Band at (*) ~ 50 kDa and at ~ 26 kDa (&) was excised and subjected to MALDI TOF-TOF analysis. (#) represents for HDAC8 in coomassie gel at 43 kDa, further confirmed by immunoblot. b MS analysis and Mascot search results confirmed TUBA1-C (Human Alpha Tubulin chain 1 C isoform) with highest topscore as the interacting partner of HDAC8. c Confirmation of HDAC8 and Alpha tubulin by immunoprecipitation using HDAC8 antibody in HeLa and HEK293T cells. d Reverse IP using Tubulin antibody confirms HDAC8 & Tubulin interaction in HeLa cells and HEK 293 T respectively

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Cell Communication and Signaling

ISSN: 1478-811X