Fig. 2

SW480 and SW620-derived EV uptake in THP-1 monocytes and M0 macrophages. a Flow cytometry analysis showing concentration-dependent uptake of Syto RNA select labelled EVs by THP-1 monocytes. The graphs show the percentage of Syto RNA select-positive THP-1 monocytes in relation to EV concentration (left) and Syto RNA Select-positive THP-1 monocytes (M, middle) and M0 macrophages (M0, right) following incubation with Syto RNA Select labelled EV at final concentration 10 μg/mL. Data are shown as mean ± SD (n = 5). Statistical analysis was carried out with one-way ANOVA test. b Representative flow cytometry histograms showing Syto RNA Select labelled SW480 and SW620 EV uptake in THP-1 monocytes and M0 macrophages (n ≥ 4). Grey lines represent untreated cells; black lines represent SW480 or SW620 EV (10 μg/mL) treated monocytes (M) or macrophages (M0). Histogram bar shows the percentage of Syto RNA select positive cells in the respective analysis. c Representative fluorescence microscopy images showing Syto RNA select labelled SW480 EV and SW620 EV uptake in THP-1 monocytes (n = 3). THP-1 monocytes were incubated with Syto RNA select labelled SW480 or SW620 EVs (10 μg/mL) for 1 h (green). The cytoskeleton was labelled with F-actin probe ActinRed 555 (red). The nuclei were stained with Hoechst 33,342 (blue). Scale bar is 10 μm