Fig. 8
From: miR-148b-3p functions as a tumor suppressor in GISTs by directly targeting KIT
Overexpression of KIT reversed the inhibitory effect of miR-148b-3p on the proliferation, migration and invasion of GIST cells. (a) GIST882 cells co-transfected with pcDNA3.1-KIT and miR-148b-3p were measured for cell proliferation by the CCK8 assay. (b) The migratory capacities of GIST882 cells co-transfected with pcDNA3.1-KIT and miR-148b-3p were assessed by transwell migration assay. (c) The invasive capacities of GIST882 cells co-transfected with pcDNA3.1-KIT and miR-148b-3p were determined by invasion chamber assay. (d) The migratory ability of GIST882 cells co-transfected with pcDNA3.1-KIT and miR-148b-3p were investigated by wound healing assay. (e) The protein expression of phosphorylated and total ERK, AKT and STAT3 in GIST882 cells was assessed by western blot analyses. *P<0.05, **P<0.01