Table 1 Summary of PCR/qPCR primer pairs
Name | PCR/ qPCR | Mean product size | Primer | Sequence (5′ to 3′) |
|---|---|---|---|---|
MMP9 | qPCR | 106bp | forward | TTCCAAACCTTTGAGGGCGA |
reverse | CAAAGGCGTCGTCAATCACC | |||
ICAM1 | qPCR | 149bp | forward | GGTAGCAGCCGCAGTCATAA |
reverse | GATAGGTTCAGGGAGGCGTG | |||
CCL2 | PCR | 297bp | forward | GCTCAGCCAGATGCAATCAATG |
reverse | GTGTCTGGGGAAAGCTAGGG | |||
CLDN1 | qPCR | 118bp | forward | CTGTCATTGGGGGTGCGATA |
reverse | CTGGCATTGACTGGGGTCAT | |||
ADAMTS9 | PCR | 301bp | forward | TTAATCTCACCGCCAATGC |
reverse | GCGCTGCGCCTATAAATGAT | |||
VEGFC | PCR | 320bp | forward | CATGTACGAACCGCCAG |
reverse | TTGGCTGTTTGGTCATTGGC | |||
IL4I1 | PCR | 451bp | forward | TCACCAAGAGCTGGAGACACC |
reverse | AACTTGGTCAGGTTGAGCCC | |||
IL7R | PCR | 281bp | forward | TAATAGCTCAGGGGAGATGGAT |
reverse | CTTGCAGAAAACCTTCCACTTCA | |||
TNFAIP3 | PCR | 629bp | forward | CAACTGAAACGGGGCAAAGC |
reverse | GCCGTCACCGTTCGTTTTC | |||
β-actin | PCR | 733bp | forward | CCTCGCCTTTGCCGATCC |
reverse | GGCCATCTCTTGCTCGAAGT | |||
GAPDH | qPCR | 87bp | forward | TGCACCACCAACTGCTTAGC |
reverse | GGCATGGACTGTGGTCATGAG |