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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Inhibition of CREB binding protein-beta-catenin signaling down regulates CD133 expression and activates PP2A-PTEN signaling in tumor initiating liver cancer cells

Fig. 4

ICG001 treatment activates PTEN and inactivates AKT and beta-catenin in murine TICs: a Western blot showing PhosphoSer380/Thr382/383-PTEN and total PTEN in ICG001 treated and DMSO control samples. b Western blot of PhosphoSer473-AKT and total AKT in ICG001 treated and DMSO controls. Protein band intensity was calculated by densitometry quantification by Image J Software, NIH. Results represent mean ±S.E from four independent experiments. (n = 4, #p < 0.005). Data was normalized to DMSO controls and represented as relative expression fold. c Western blot analysis for pSer552-beta-catenin in ICG001 treated samples and DMSO controls. Data was normalized to DMSO controls and represented as relative expression fold (n = 4 *p < 0.05). d Immunofluorescence staining for phospho-Ser552-beta-catenin in DMSO and ICG001 treated TICs. Isotype IgG staining was performed as staining control. Number of phospho-Ser552-beta-catenin positive cells per high power field (HPF) was manually counted and compared to DMSO control. Results represent mean ±S.E from four independent experiments (n = 5, *p < 0.01). Scale bar = 25 µm

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Cell Communication and Signaling

ISSN: 1478-811X