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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

Fig. 2

Co-culture with adipocytes induces ATGL-dependent lipolysis in breast cancer cells. a Expression of ATGL in mammary epithelial cell lines using western blotting. b After co-culture with mature adipocytes for 3 days, expression of ATGL in SK-BR-3 and SUM159PT cells was obviously increased. c Removing adipocytes and depriving serum from the medium led to lipolysis in cancer cells, and the TG content in tumor cells was detected at 0, 4 and 24 h after release, while free glycerol in the medium was detected at 4 and 24 h. d Down-regulation of ATGL in SUM159PT cells by shRAN plasmid transfection and validation of the effects using western blotting. Proliferative ability was compared between shCtrl and shATGL stably expressed SUM159PT cells (left panel). Transwell assays conducted to elucidate the impact of ATGL blockade on the migration ability of cancer cells. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, “ns” stands for not significant

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Cell Communication and Signaling

ISSN: 1478-811X