Fig. 4

Effect of HQGGT on TS expression. Cells were treated with HQGGT (3 mg/mL) for 48 h followed by processing for immunoblot (a) and qRT-PCR (b) analysis. Basal TS expression in CRC cell lines was also determined by immunoblot analysis. HT-29 (c) and MC38 (d) cells were treated with HQGGT for 48 h and processed for immunoblot analysis. e HT-29 cells were treated with two different batches of HQGGT for 48 h and processed for immunoblot analysis. f HT-29 cells were treated with different HQGGT batches (3 mg/mL) for 12, 24, 36, and 48 h, respectively, and processed for immunoblot analysis. TS protein expression was quantified by ImageJ and normalized by GAPDH expression. Values represent the mean ± SD from 3 independent experiments. *, p < 0.05, **, p < 0.01, versus untreated control