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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Herbal formula Huang Qin Ge Gen Tang enhances 5-fluorouracil antitumor activity through modulation of the E2F1/TS pathway

Fig. 2

HPLC profile of two batches of HQGGT and effect of HQGGT on cell cycle distribution. a The chemical fingerprint of HQGGT batch #1 and #2 was measured by HPLC analysis. b HCT116 cells were treated with HQGGT (3 mg/mL) for 48 h, followed by fixation, PI staining, and cell cycle analysis by flow cytometry (left panel). The percentage of HCT116 cells in sub G0, G0/G1, S, G2/M phases (middle panel) and the percentage of HT-29, RKO and H630R1 cells in sub G0 phase are shown (right panel). Values represent the mean ± S.D. from three independent experiments. *, p < 0.05; **, p < 0.01, versus untreated. c HT-29, RKO, HCT116 and H630R1 cells were treated with HQGGT (3 mg/mL) for 48 h and processed for immunoblot analysis. HT-29 cells were treated with various doses of HQGGT (1, 2 and 4 mg/mL) for 48 h. The expression of cleaved PARP was analyzed by immunoblot analysis and a representative image from three individual experiments is shown

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