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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: LncRNA HOTAIR promotes cell migration and invasion by regulating MKL1 via inhibition miR206 expression in HeLa cells

Fig. 7

Detect the localization of HOTAIR and miR-206 by RNA FISH in HeLa cells. a Detect the localization of HOTAIR and miR-206 by RNA FISH in HeLa cells. The HOTAIR probes were labelled with Alexa Fluor 647 at the 5 terminal. And the probe of miR-206 was labelled with FAM. Nuclei are stained blue with DAPI. b The efficiency of HOTAIR knockdown was determined after transfecting siRNA targeted against HOTAIR in HeLa cells at 48 h by qRT-PCR. The expression level of HOTAIR was normalized to GAPDH. HeLa cells were transfected with siHOTAIR or siNC for 48 h. c. The RNA expression of miR-206 after transfecting siHOTAIR at 48 h by qRT-PCR. The expression level of miR-206 was normalized to U6. Data are presented as means ± S.D. and results are from one representative experiment of at least three. *, P < 0.05; **, P < 0.01 (Student’s t test)

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