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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: LncRNA HOTAIR promotes cell migration and invasion by regulating MKL1 via inhibition miR206 expression in HeLa cells

Fig. 5

miR-206 degraded MKL1 by targeting the specific sites. a HeLa cells were transfected with miR-206 mimics or NC for 48 h. The abundance of miR-206 expression was determined by qRT-PCR. The expression level of miR-206 was normalized to U6. b Western blots analysis of the MKL1 protein expression at 48 h after transfected with miR-206 mimics or NC. GAPDH was used as an internal control. c qRT-PCR was used to measure the efficiency of miR-206 inhibition after transfecting miR-206 inhibitor at 48 h. The expression level of miR-206 was normalized to U6. d Western blots analysis of the MKL1 protein expression at 48 h after transfected with miR-206 inhibitor or NC. GAPDH was used as an internal control. e Diagram of MKL1–3’UTR containing reporter constructs. The seed sequence of miR-206 was underlined. Mutation contains 4-base-mutation at the miR-206 targeting region, abolishing its binding. f. In luciferase assays using HeLa cells, transfection of miR-206 and MKL1–3’UTR-mut increased the luciferase activities compared with transfection of miR-206 and MKL1–3’UTR-WT. Data are presented as means ± S.D. and results are from one representative experiment of at least three. *, P < 0.05; **, P < 0.01 (Student’s t test)

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