Fig. 7

In vivo chemotherapeutic responsiveness of MDA-hyb1 cells. In vivo chemotherapeutic response to 25 mg/kg taxol was tested after subcutaneous injection of 2 × 10⁶ MDA-MB-231^GFP cells or 2 × 10⁶ MDA-hyb1^cherry cells into 6 NODscid mice, respectively (3 mice for control and 3 mice for taxol-treatment). Based on an average weight of 20 g/mouse gavage was performed with 0.5 mg/200 μL taxol twice weekly and 200 μL solvent in control mice starting 4d after tumor cell application. The twelve mice were euthanized after 22d (MDA-Hyb1^cherry) and 26d (MDA-MB-231^GFP) and dissected tumors were quantified for tumor size and analyzed for distant organ metastases by fluorescence microscopy (n.d. = not detectable)