Fig. 6
Tumor and metastases formation of MDA-hyb1 and MDA-hyb2 cells. a Quantification of the tumor weight was performed after dissection of the solid subcutaneous primary tumors only (disregard of metastatic tumor tissue) derived from the parental MDA-MB-231GFP cells (MDA-GFP) in comparison to mcherry-labeled MDA-hyb1 and mcherry/GFP-labeled MDA-hyb2 populations. Data were obtained by the mean ± s.d. from 5 different tumors of each cell type after 18d. Moreover, additional 4 different MDA-GFP tumors were analysed after 34d. Significance was calculated by student’s-t-test. b Quantification of the relationship of tumor weight / mouse weight was calculated with tumors of the parental MDA-MB-231GFP cells (MDA-GFP) in comparison to mcherry-labeled MDA-hyb1 and mcherry/GFP-labeled MDA-hyb2 populations. Data were obtained from 5 different tumors of each cell type after 18d. Moreover, additional 4 different MDA-GFP tumors were analysed after 34d. Significance was calculated by student’s-t-test. c Organs from four mice from each injected tumor cell population were dissected after euthanization and evaluated by fluorescence microscopy whereby appropriate fluorescence should indicate formation of distant metastases. Appearance of organ metastases from all of these 16 mice was quantified and exemplary pictures from tissue thin section phase contrast / fluorescence microscopy of organ metastases were documented. n.d. = not detectable. Bars represent 100 μm