Fig. 5
Effects of S100A4 knock-down in hybrid cell formation and in vivo tumor development of hybrid cells. a Efficiency and significance of hybrid cell formation by MDA-MB-231cherry and MSCGFP fusion was examined and quantified following siRNA targeted down-modulation of S100A4 versus a non-targeting siRNA. The relative amount of hybrid cells was calculated by the mean ± s.d. from 3 different co-cultures and significance was calculated by student’s-t-test. b In vivo tumor development was tested after simultaneous subcutaneous injection of 2 × 106 cells of MDA-MB-231GFP, MDA-hyb1, or MDA-hyb2 cells into 5 NODscid mice respectively. The fifteen mice were euthanized after 18d and dissected tumors were compared for tumor size. In addition, 2 × 106 cells of MDA-MB-231GFP cells were injected subcutaneously into 4 NODscid mice. to achieve a comparable tumor size like MDA-hyb1 cells after 34d