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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Enhanced metastatic capacity of breast cancer cells after interaction and hybrid formation with mesenchymal stroma/stem cells (MSC)

Fig. 3

Microarray and PCR analysis of the two hybrid populations in comparison to the parental cell lines. a Quantification of genes with changed expression levels (up-regulation = numbers in upper rectangular; down-regulation = numbers in lower rectangular) in MDA-hyb1 (upper panels, red and green) and MDA-hyb2 cells (lower panels, red and green) was performed following microarray analysis with comparison to the parental MDA-MB-231cherry cells (red panels) and to the parental MSC051212GFP (green panels) respectively. Changes in expression levels of more than 2-fold were considered as up- or down-regulated. b PCR analysis was performed with mcherry, eGFP, fusion-associated factors syncytin-2 and MFSD-2A, and MSC stem-like markers CD73, CD90, and CD105 expression in the parental MDA-MB-231 and MSC populations as compared to the two hybrid populations. Unaltered GAPDH transcripts served as loading control. c Comparison of EMT-related gene expression in MDA-hyb1 relative to MDA-MB-231 (orange-colored bars) and in MDA-hyb2 relative to the parental MDA-MB-231 cells (kaki-colored bars). A variety of genes are up-regulated during EMT (upper panel) in parallel to a down-modulation of other genes (lower panel) to enable development of a mesenchymal phenotype

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