Fig. 5
Effect of GANT61 on motility and invasion of breast cancer cells. MDA-MB-231 and MCF-7 were treated with the conditional medium containing 10Ā Ī¼M GANT61 and the control medium. a. Migration was assessed using scratch assay and readings were taken after every twelve hour for 48Ā h, upper panel of both cell lines represent 0Ā h and the lower panel represents 48Ā h. b. Graph showing difference of distance migrated between untreated and treated cells at each time point of scratch assay. c. Invasion was assessed using Boyden chamber transwell assays and difference between treated and untreated cells was evaluated after 24Ā h. d. Graphical representation of number of cells invaded as meanāĀ±āS.D. between untreated and treated cells in both cell lines. (Unpaired t test, ***, ###pā<ā0.0001). All experiments were conducted in triplicates and were performed thrice