Fig. 6
From: A functional role of LEFTY during progesterone therapy for endometrial carcinoma
LEFTY is a GSK-3β substrate. a Structure of LEFTY1/2 protein showing the positions of five putative GSK-3β phosphorylation motifs. The corresponding constructs that were used in this study are also shown. wt, wild-type; mut, mutant type. b After treatment of ES-2 cells stably overexpressing LEFTY1 with 10 μM MG132 for 5 h with or without 40 mM LiCl for 24 h (upper: input), LEFTY (indicated by arrow) was immunoprecipitated (middle), and then ubiquitination of LEFTY (indicated by arrowhead) was examined (lower). c Western blot analysis for the indicated proteins after treatment of ES-2 cells stably overexpressing LEFTY1wt-HA or LEFTY1 mt (5SA)-HA with 40 mM LiCl for 24 h