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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: NFAT1-regulated IL6 signalling contributes to aggressive phenotypes of glioma

Fig. 3

IL6R knockdown inhibits the growth and invasion of glioma cells. a IL6R protein levels in M1 and U87 cells that were transfected with IL6R-shRNA1, IL6R-shRNA2 or control-shRNA, respectively, were tested by western blot analyses, and IL6R knockdown was confirmed. b Targeting of IL6R via specific shRNAs significantly decreased the proliferation of M1 and U87 cells as assessed with MTS assay. c Representative microphotographs showing the detection of apoptosis using the TUNEL assay in control and IL6R silenced M1 cells. Scale bar = 25 μm. Histogram showing the quantification of the apoptotic rate. d Representative microphotographs showing the invasion of M1 and U87 cells in the presence of IL6R-shRNA or control-shRNA using the matrigel assay. Scale bar = 50 μm. Histogram showing the quantification of invasive cells. e The migration of M1 and U87 cells in the presence of IL6R-shRNA or control-shRNA was tested using the wound healing assay. Scale bar = 500 μm. Histogram showing the quantification of the migration rate. f IL6R knockdown decreased the colony formation rate of M1 and U87 cells. Scale bar = 50 μm. g Representative images of the effect of IL6R knockdown on M1 and U87 GSC neurospheres. Scale bars = 50 μm. Histograms showing the quantification of relative sizes and numbers of indicated neurospheres. Results are presented as mean ± SEM of triplicate samples from three independent experiments. *P < 0.05 and **P < 0.01

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