Fig. 1

SLC9A9 transcript is a target of miR-135a-5p and miR-124-3p. a Seed sequences of miR-135a-5p, miR-124-3p and miR-153-3p along with their predicted binding sites in SLC9A9 3’-UTR are shown. The highlighted regions represent the binding sites as predicted by TargetScan. b MiR-135a-5p and miR-124-3p target the predicted binding sites within the transcript of SLC9A9 3’-UTR, decreasing luciferase activity in HEK293 cells transfected with a luciferase reporter. Relative luciferase activity normalized to protein concentration is plotted. c Luciferase activity as described in (b) is shown for U87 cells (d) Luciferase activity as described in (b) is shown for U251n cells. Error bars represent standard deviation (SD) determined from at least three biological replicates; *p < 0.05. Statistical analysis was done using student’s t-test