Fig. 6

AMPK-mTOR-S6 K1-S6 signaling regulates growth and migration of HCC cells. a-g, Cells were transfected with indicated shRNA for 24 h, then cells were treated with or without C-C, followed by testing migration,growth,proliferation and apoptosis. (a) migration ability was analyzed by wound healing assay. Representative images (left) and relative migration distance (right) was plotted. Migration distance in shScr group at 6 h was set as 1. N = 4, **, p < 0.005, NS, no significant. (b) cell migration was analyzed by trans-well. Representative images (left) and relative migration cell numbers (right panel) were plotted. Migration cell numbers in shScr group was set as 1. N = 4, **, p < 0.005, NS, no significant. (c) Relative cell numbers at 4 days after transfection was plotted. N = 4, *, p < 0.05,**, p < 0.005,***, p < 0.001,NS, no significant. (d) cell numbers at indicated days after transfection was plotted. N = 4, ***, p < 0.001,NS, no significant. (e) cell proliferation was determined by Hoechst and EdU labeling assay. Representative images were shown. EdU-labeled (pink) indicated proliferation cells. (f) the proliferation rate was plotted. N = 4, **, p < 0.005, NS, no significant. (g) the apoptosis percentage was plotted. N = 3, **, p < 0.005, NS, no significant. h-j, HepG2 cells were infencted with ATIC shRNA for 24 h, then were infected with AMPK shRNA for 48 h. (h), cell migration was determined by trans-well assays. Representative images (left) and relative migration cell numbers (right) were plotted. Migration cell numbers in shScr group was set as 1. N = 4, **, p < 0.005, NS, no significant. (i), Cell numbers at indicated days after transfection was plotted. N = 4, ***, p < 0.001,NS, no significant. (j), Cell apoptosis was determined by flow cytometry. The apoptosis percentage was plotted. N = 3, **, p < 0.005, NS, no significant