Fig. 1

ATIC is up-regulated in HCC patients. a, RT-PCR analysis shows the mRNA level of ATIC in 12 pairs of HCC cancers and the adjacent non-cancerous liver tissues. Overexpression of ATIC was observed in 11 out of 12 HCC patient samples. ATIC mRNA expression level in HCCs and non-cancerous tissues were normalized to GAPDH. Experiments were repeated three times, Values represent mean ± SD. b, the protein level of ATIC was analyzed by Western blot in 12 representative pairs of HCC tumors and the adjacent non-cancerous liver tissues. A representative of three experiments is shown. N, Non-cancerous; C, Cancer. c, the relative level of ATIC protein was quantified using Image J. Fold change of ATIC protein with respect to non-cancerous specimens was normalized to GAPDH. Values represent mean ± SD, N = 3. d, Log2-transformed fold changes of ATIC mRNA expression level in liver normal and tumor tissues. Data from GENT database. (mean ± S.D., Unpaired t-test; U133plus2 data set, Cancer N = 182, Normal N = 25; U133A data set, Cancer N = 156, Normal N = 52.) e, ATIC mRNA levels in indicated samples. Data from GENT U133Plus2 data set. Normal N = 8, early HCC N = 18, advanced HCC N = 17. f, ATIC mRNA levels in HCC samples with different TNM stage. TNM Stage I N = 172, II N = 86, III-IV N = 90. Data from TCGA database. g, ATIC mRNA levels in HCC samples with different histologic grade. Histologic grade I N = 55, II N = 178, III-IV N = 134. Data from TCGA database. h, Expression of ATIC in indicated cell lines and adjacent non-cancerous liver tissue from patient #1 was analyzed by western blot