Fig. 6
From: Sirt1 interaction with active Smad2 modulates transforming growth factor-β regulated transcription
Sirt1 accumulates on Smad regulating elements in response to TGFβ treatment in HaCaT cells. Cells were kept overnight in OPTIMEM culture medium and stimulated with TGFβ for 1.5 h. a and b. ChIP analysis of the SBR and TSS regions of the JUN gene (a) and the SBR and TSS regions of the PAI-1 (b) gene using antibodies against RNA PolII, phosphorylated-Smad2/3, Sirt1 and control IgG. Shown data are means and standard deviations of qPCRs performed in triplicate in a representative experiment