Fig. 3
From: Sirt1 interaction with active Smad2 modulates transforming growth factor-β regulated transcription
The interaction occurring between Sirt1 and Smad2 is domain-specific. a Schematic representation of full length GST-Smad2 and GST-Smad3 and different GST-Smad2 fragments. b A His-Pull-down assay was performed to determine interaction between Sirt1WT-6xHis and full-length GST-Smad2 or GST-Smad3, or GST-Smad2 fragments. Different constructs were detected by Western Blot. c Schematic representation of full length Sirt1WT-6xHis and different Sirt1-6xHis fragments. “CD”: catalytic domain. d His-Pull-down assay to determine interaction between Flag-Smad2 and Sirt1-6xHis and different Sirt1-6xHis fragments. Flag-Smad2 was expressed in HEK293T cells and purified with a flag peptide. Different constructs were detected by Western Blot. All the experiments of this figure were repeated at least three times. Representative results are shown