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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities

Fig. 2

Overexpression of HAS1 induces Epithelial-to-Mesenchymal Transition (EMT) in MCF10A cells. a The normal mammary cell line MCF10A was mock transfected (left), or transfected with a plasmids that express HAS1 (middle) or an unrelated protozoa gene LMA2 (right). The selected populations were cultured in 3D reconstituted basement-membrane model. Both control panels (right and left) show acini structure in contrast to middle panel. b MCF10A cells were transfected as described in (a) and subjected to EMT analysis using the common EMT markers E-cadherin (Epithelial) and N-cadherin (Mesenchymal) using RT-qPCR. The relative expression of E-cadherin is diminished, however N-cadherin is over-expressed in MCF10A-HAS1 cells

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Cell Communication and Signaling

ISSN: 1478-811X