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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities

Fig. 1

Overexpression of epitope-tagged human HAS1 increases cytoplasmic HA concentration in MCF10A cells. a Non-tumorogenic mammary cell line (MCF10A) expressing HAS1 shows significantly more HA staining (green) in comparison to protozoa-gene transfected MCF10A-LMA2 (control). The punctate localization of HAS1-synthesized HA inside the cell was observed. MCF10A-HE-HAS1: HAS1 in pCDNA3 with N-terminal hemagglutinin fusion-tag, MCF10A-A2-HAS1: HAS1 in pCDNA3 with N-terminal A2 fusion-tag, and MCF10A-LMA2: a Leishmania major (protozoa) gene (Lm2415) in pCDNA3 with C-terminal A2 fusion tag. The HAS1 in pCEP4 with N-terminal A2 fusion-tag is shown as ‘MCF10A-A2-HAS1 (in pCEP4). b MCF10A-A2-HAS1 and MCF10A-HE-HAS1 cells are brightly stained for HA (green) and CD44 (red) in comparison to MCF10A-LMA2 cells. The cells indicated in (A) were subjected to both HA and CD44 immunofluorescence staining. The middle panel shows the bright-field image to identify the edge of the cells. Nuclei were stained with DAPI (blue). Multiple focused- or Z-stacking images were used to compile these composite images for complete top-vision across the thickness of the cells

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Cell Communication and Signaling

ISSN: 1478-811X