Fig. 4
From: Impaired mitochondrial calcium uptake caused by tacrolimus underlies beta-cell failure
Tacrolimus at therapeutic dosage reduces mitochondrial ATP production. The enzymatic activity of cytochrome c oxidase was measured in beta-cells (at least 30 per group) incubated for 24 h with vehicle or 5 nM Tacrolimus (a); data represent mean ± S.E.M. The ATP/ADP ratio in response to 16.7 mM glucose was determined by PercevalHR ratio and corrected by pHRed ratio (b); the peak amplitude is quantified in panel c, where whiskers represent 1% to 99% spread of the data; *:p < 0.01 vs vehicle