Fig. 1

Therapeutic doses of tacrolimus alter insulin secretion without affecting cell viability. Insulin release measured in beta-cells incubated for 24 h with vehicle or tacrolimus at the indicated doses (a) and with vehicle or 5 nM tacrolimus at the indicated times (b). Effect of fuel secretagogues leucine (Leu, 10 mM) and glutamine (Gln, 2 mM) on beta-cells treated for 24 h with vehicle or 5 nM tacrolimus (c). Cell viability assessed in beta-cells treated for 24 h with vehicle or 5 nM tacrolimus (d). Data are presented as mean ± S.E.M. of experiments performed at least in triplicate; *:p < 0.01 vs vehicle, ANOVA. In panel d, whiskers represent 1% to 99% spread of the data; NS: non-significant; t test