Purpose of experiment | Pore size of the transwell filter | Reference | Effect of the transwell filter | Observations/Conclusions |
---|---|---|---|---|
Transfer of HIV-1 virions between Jurkat T cells | 3000 nm | [14] Sowinski et al., 2008 | Negative | Decreased HIV-1 transfer |
Transfer of apoptotic signal from stromal cells to B cells | 3000 nm | [15] Teague et al., 2010 | Negative | Cell contact was necessary for transfer |
Transfer of miRNAs between cancer and endothelial cells | 3000 nm & 400 nm | [2] Connor et al., 2015 | Negative | Transfer of miRNA was reduced to basal levels |
Lysosomes mediated transfer of cystinosin & cystinebetween mouse fibroblasts and macrophages | 1000 nm | [7] Naphade et al., 2015 | Negative | Decreased cysteine transfer |
Transfer of DiD between NRK, CHO and HeLa cells | 450 nm | [13] Schoelermann et al., 2015 | Negative | Transfer of DiD was reduced |
Transfer of CD40L-induced cytoplasmic and cell surface–associated material from T cells to dendritic cells | 450 nm | [17] Zaccard et al., 2014 | Negative | Decrease in transfer |
Transfer of prion particles between mouse neuronal cells | 400 nm | [4] Gousset et al., 2009 | Negative | Prion transfer was entirely abolished |
Transfer of cues for cell migration from endothelial cells to bronchial epithelial cells | 400 nm | [18] Zani et al., 2010 | Negative | Bronchial epithelial cell migration required direct cell contact with endothelial cells |
Transfer of prion particles in mouse neuronal cells | 400 nm | [5] Langevin et al., 2010 | Negative | Prion transfer was reduced over 98% |
Transfer of mitochondria from vascular smooth muscle cells to mesenchymal cells | 400 nm | [16] Vallabhaneni et al., 2012 | Negative | Direct cell contact was required for mitochondrial transfer |
Transfer of p-glycoprotein between MCF-7 breast cancer cells | 400 nm | [42] Pasquier et al., 2012 | Negative | Reduced p-glycoprotein transfer |
Transfer of Huntington mutant protein (Htt) aggregates in mouse neuronal cells | 400 nm | [3] Costanzo et al., 2013 | Negative | Htt aggregate transfer was blocked by over 95% |
Transfer of contact dependent proliferation cues from astrocytes to glioma cells | 400 nm | [19] Zhang and Zhang, 2015 | Negative | Decreased transfer of molecular cues |
Transfer of mCherry from senescent cells to NK cells | 400 nm | [1] Biran et al., 2015 | Negative | mCherry transfer was reduced |
Transfer of soluble amino acids between bacterial cells | 200 nm | [9] Pande et al., 2015 | Negative | Amino acid transfer was entirely blocked |
Mitochondrial transfer in MDA-MB231, OVCAR3, SKOV3 and MCF-7 cells | NA | [42] Pasquier et al., 2013 | Negative | Decrease in mitochondrial transfer |