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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: CD146, a novel target of CD44-signaling, suppresses breast tumor cell invasion

Fig. 1

Western blot and immunocytochemical analyses demonstrating the inverse relationship between CD44 and CD146 expression. a MCF7-B5-CD146 cells (B4 cells) in the absence (−Dox) or presence (+Dox) of induced CD44 expression were activated with the CD44 ligand, Hyaluronan (HA). Total cell extracts were made and the presence of CD44, CD146, or beta-actin (loading control) at 0, 24, 48 h was determined by Western blot analysis. Shown are the cropped blot images representing indicated proteins, and all the gels from three separate experiments have been run under the same experimental conditions. b Immuno-fluoresence staining of CD146 in MCF7-B5-CD146 cells, indicates the upregulation of CD146 expression at the cell surface in the absence of CD44 (Dox +, HA-). Data was presented as Mean ± Standard deviation of at least triplicates from three separate experiments. The difference is considered statistically significant (student’s two-tailed t-test, P < 0.001)

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