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Table 1 Gene expression Analysis

From: Human umbilical cord Wharton jelly cells promote extra-pancreatic insulin formation and repair of renal damage in STZ-induced diabetic mice

Oligo name

Type

Untreated-Diabetic Mice (n = 5)

Normal Mice (n = 5)

TM-RH (n = 4)

TM-H (n = 7)

Dil-SP (n = 4)

hUC-WJCs

Fluorescent Detection

  

Pa

Ki

Li

Sp

Pa

Ki

Li

Sp

Pa

Ki

Li

Sp

Pa

Ki

Li

Sp

Pa

BT

 

Beta-Actin a

 

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

SYBR

Alu-Sequence

         

+

+

+

–

+

+

+

–

+

+

hydrolysis

POUF51

ES

x

x

x

x

x

x

x

x

x

x

–

x

x

x

x

x

–

+

SYBR

HLA-1

MSC

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

+

SYBR

HLA-DR

MSC

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

SYBR

SOX17

DE

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

 

SYBR

FOXA2

DE

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

 

SYBR

Hnf4-Alpha

PG

    

–

+

+

–

–

+

+

–

    

–

 

SYBR

HNF6

PF

     

–

1f

x

–

–

32f ± 9.1f

–

      

SYBR

PDX1

PF

x

x

x

x

x

x

x

x

x

x

x

x

    

x

 

SYBR

NKX6–1

PE

x

–

x

x

x

–

x

 

x

–

–

x

    

x

 

SYBR

Ngn3

PE

x

x

x

x

x

x

x

x

x

x

–

x

    

x

 

SYBR

NEUROD

PE

    

x

x

x

x

x

x

–

x

    

x

 

SYBR

PAX6

PE

    

x

x

x

x

x

x

+

x

    

x

 

SYBR

Ptf1a

 

x

x

x

x

x

x

x

x

x

x

x

x

    

x

 

SYBR

MafA

 

x

x

x

x

x

x

x

x

x

x

–

x

    

x

 

SYBR

VEGF-A

     

x

–

1f

x

x

–

50 ± 2.3f

–

    

x

 

SYBR

HES1

     

–

1f

1f

–

–

−500 ± 15f

−7 ± 1.5f

–

    

–

 

SYBR

Human-INS

EN

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

x

hydrolysis

Human Glucagon

EN

x

x

x

x

x

x

x

x

x

x

x

x

    

x

 

SYBR

Mouse-INS2

EN

–

x

x

x

1f

1f

1f

x

−9.8f ± 4.4f

71.3 ± 8.2f

60.4 ± 7.4f

x

x

–

–

x

x

x

hydrolysis

  1. Legend: Gene expression analysis in normal, diabetic and TM using qPCR. (MSC) Mesenchymal Stem Cells; (ES) Embryonic Stem; (DE) Definitive Endoderm; (PG) Primitive Gut tube; (PF) Posterior Foregut; (PE) Pancreatic Endoderm; (EN) Endocrine Stage; (Pa) Pancreas; (Ki) Kidney; (Li) Liver; (Sp) Spleen; (Dil-SP) Dil-stained pancreases; (BT) Before Transplantation. The gene expression was defined as highly expressed (+) when the quantification cycle (Cq) < 30; poorly expressed (−) when Cq ≥ 30 < 35; not expressed (X) when Cq ≥ 35 or when there was no transcript amplification; and () not assayed
  2. Significant differences in the gene expression between groups are expressed as fold change values. For each gene expressed as fold change in the Normal Mice group, the sample was set to 1, and the samples for comparison were normalized to this level. Positive fold change values represent upregulation. Negative values represent downregulation. Fold changes are defined directly in terms of ratios
  3. aEach marker was analyzed with SYBR Green fluorescent detection, and the transcript levels were normalized to those of the endogenous control β-actin. The data are the means ± s.d
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Cell Communication and Signaling

ISSN: 1478-811X