Fig. 3

Immunofluorescence detection and insulin quantification. Representative pictures of pancreatic islets in the different groups. Immunofluorescence detection was performed with the primary anti-insulin (C27C9) rabbit mAb, 1:400 (mouse and human cross-reactivity). In contrast to islets from normal mice (a), pancreases from TM-RH (b), TM-H (c), and untreated diabetic mice (d) presented smaller insulin-producing islets (e). Moreover, the treated groups presented fewer islets per section than normal mice (f). Arrows in (c) and (d) indicate the localization of insulin formation. Different shades of gray bars in (e) represent different mice. INS scale bar = 20 μm. The data are the means ± s.d. *P < 0.05; **P < 0.01. Area distribution was calculated with ImageJ software. The islets per section were counted manually (n = 5)