Fig. 8

LIP induces exposure of phosphatidylserine and binds phosphatidylserine. a LIP induces exposure of phosphatidylserine. Cells were treated with LIP for 30 min. The cells were then imaged using Zeiss LSM 780 inverted microscope after staining for annexin-V-FITC. b LIP induces cell death in MCF-7 cells and Jurkat cells. Cells were treated with LIP for 12 h. The cells were then subjected to flow cytometric analysis after staining for annexin-V-FITC and propidium iodide, as mentioned in the methods. Shown are the data from three independent experiments. c Effect of LIP on a liposome membrane composed of a mixture of PC and CHL containing calcein. d Effect of LIP on a liposome membrane composed of a mixture of PC and PS containing calcein. e Calorimetric measurements of the LIP interaction with PS