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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: A novel protein derived from lamprey supraneural body tissue with efficient cytocidal actions against tumor cells

Fig. 3

Cytocidal activity of recombinant LIP. a MCF-7 cells were incubated with unrelated protein purified from E.coli (rC1q), heat-denatured LIP (0.5 μg/mL) and recombinant LIP (0.5 μg/mL) for 12 h at 37 °C. Cell death was analyzed by PI staining and by flow cytometry. PBS-treatment cells were used as a negative control (left pane). Histogram showing statistics of the above results (right pane). All experiments were repeated at least three times with similar results. b The cells were incubated with 0.5 μg/mL LIP at 37 °C and visualized by Live-Cell Imaging. c Cytocidal activity of LIP against cultured tumor cells. A total of 2 × 104 cells were preincubated at 37 °C for 20 h and then treated with LIP (final concentrations, 0.2 μg to 2 μg) for 12 h at 37 °C. Cytocidal activity of LIP was determined using the LDH cytotoxicity assay kit

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