Fig. 1

Identification of active protein from supraneural body in the lamprey. a Tissue of the supraneural body by hematoxylin and eosin (left pane). Types of cells from the supraneural body (right pane). b Macro-Prep Ceramic Hydroxyapatite column of flow-through and eluted fraction. – green line, KPB gradient; − blue line, absorbance at 280 nm; □ activity protein(upper pane). Q Sepharose Fast Flow column of flow-through and eluted fraction. – green line, KCl gradient; − blue line, absorbance at 280 nm;□ activity protein (low pane). c MCF-7 cells were seeded at 5 × 10⁴ cells in a 96-well plate. Each of the purified fractions were added to MCF-7 cells, and photographs were taken after the 30 min using optical microscope. (Magnification: 100×). d Determination of the purity and molecular mass of LIP via SDS-PAGE under reduced conditions. Lane 1, protein molecular markers; lane 2, native active LIP. e Identification of the molecular weight of LIP through MALDI-TOF mass spectroscopy