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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Impairment of IGF2 gene expression in prostate cancer is triggered by epigenetic dysregulation of IGF2-DMR0 and its interaction with KLF4

Fig. 6

Epigenetic status of IGF2-DMR0 and expression of KLF4 in prostate cancer cell lines LNCaP and DU145. a Pyrograms show that LNCaP cells are hypomethylated in IGF2-DMR0 (average 17%), whereas DU145 cells are hypermethylated (average 85%); b Analysis of post-translational histone modification (PTHMs) by chromatin immunoprecipitation revealed an enrichment of H3K9me3 and H3K27me3 and a depletion of H3K4me3 in IGF2-DMR0 of LNCaP cells. In contrast, in DU145 cells an enrichment of H3K4me3 and a depletion of H3K9me and H3K27me3 was found; c Expression of KLF4 was confirmed in LNCaP and DU145 at mRNA level by RT-qPCR (c.1) and at protein level by western blot (c.2, GAPDH was used as control protein)

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