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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Impairment of IGF2 gene expression in prostate cancer is triggered by epigenetic dysregulation of IGF2-DMR0 and its interaction with KLF4

Fig. 3

Association of IGF2 LOI to IGF2 gene expression and DNA methylation in IGF2-DMR0 and IGF2/H19-ICR. a IGF2 imprinting status (one or two allele expression) was analyzed by restriction fragment length polymorphism (RFLP, ApaI, GGGCC^C) on cDNA generated from tissue samples with heterozygous genotypes. A representative RFLP agarose gel image shows two cases with LOI (loss of imprinting; samples 29 and 44 show bi-allelic IGF2 expression) and five cases with MOI (maintenance of imprinting; samples 24, 26, 39, 42 and 37 show one-allelic IGF2 expression); b No significant difference regarding IGF2-mRNA expression was found between prostate tissue samples exhibiting LOI and MOI; c Methylation of IGF2-DMR0 was not significantly changed in samples exhibiting LOI in comparison to those with MOI; d A significantly higher methylation in IGF2/H19-ICR was found in LOI-group. P-values were determined by Mann-Whitney U test

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