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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Impairment of IGF2 gene expression in prostate cancer is triggered by epigenetic dysregulation of IGF2-DMR0 and its interaction with KLF4

Fig. 2

Expression of IGF2-mRNA as a function of DNA methylation in IGF2-DMR0 and IGF2/H19-ICR. a IGF2-mRNA expression was measured by RT-qPCR in TUR-BPH (benign prostate hyperplasia obtained by transurethral resection of the prostate), RP-BPH (BPH adjacent to prostate carcinoma, PCa, obtained by radical prostatectomy) and RP-PCa samples (PCa obtained by radical prostatectomy). A significantly reduced IGF2-mRNA expression was detected among RP-BPH and RP-PCa; b DNA methylation in IGF2-DMR0 was measured by pyrosequencing in TUR-BPH, RP-BPH and RP-PCa. A significant hypomethylation of IGF2-DMR0 was observed particularly in RP-PCa samples in comparison to TUR-BPH. In RP-PCa samples the highest variance of IGF2-DMR0 methylation was observed; c The methylation levels of IGF2/H19-ICR were not significantly different among the three analyzed groups. A relative high deviation of IGF2/H19-ICR methylation was found in RP-BPH and RP-PCa samples. P-values were determined by Mann-Whitney U test

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