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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: NF-κB potentiates tumor growth by suppressing a novel target LPTS

Fig. 7

NF-κB promotes LPTS-dependent growth of cancer cells. a Relative mRNA Expression of human P65 and LPTS in the normal or primary tumor tissues in the colon. Data were obtained from an open database (http://merav.wi.mit.edu/SearchByGenes.html). The normal tissues include 38 samples and the tumor tissues include 716 samples. b Relative mRNA levels of human P65 and LPTS in the normal or primary tumor tissues in the stomach. Data were collected from the open database as described in (a). The normal tissues include 10 samples and the tumor tissues include 15 samples. c Immunoblotting assays of P65 in MC-38 cells. The PLKO or a mouse p65 shRNA (mP65-KD)-transfected MC-38 cells were additionally transfected with a vector control or a mouse LPTS shRNA (mLPTS-KD). The cells were harvested for western blotting assays. d mRNA expression of LPTS in MC-38 cells as described in (c) (n = 4, **P < 0.01, means ± s.e.ms., Student’s t-test). e CCK8 assays were performed on the MC-38 cells described in (c). (n = 5, **P < 0.01, means ± s.e.ms., Student’s t-test). ns, not significant. f Volume of the inoculated MC-38 tumors. The MC-38 cells described in (c) were subcutaneously inoculated in C57BL/6 mice and the tumor size was determined dynamically. (n = 5, *P < 0.05, means ± s.e.ms., Student’s t-test). ns, not significant. g Percent survival of the MC-38 tumor-bearing mice. The C57BL/6 mice were inoculated with subcutaneous MC-38 tumors as described in (f), and the survival time was recorded. (n = 10, **P < 0.01, means ± s.e.ms., Gehan-Breslow-Wilcoxon test). ns, not significant. h p65 silence inhibited gastric cancer cell growth in a LPTS dependent manner. The PLKO or P65-shRNA-2-transfected MKN-45 cells were additionally transfected with a vector control or a human LPTS shRNA (hLPTS-KD). The cell growth was measured by CCK8 assays. (n = 5, **P < 0.01, means ± s.e.ms., Student’s t-test). ns, not significant. i Volume of the inoculated MKN-45 tumors. The MKN-45 cells described in (h) were subcutaneously inoculated in rude mice and the tumor size was determined dynamically. (n = 5, ***P < 0.005, means ± s.e.ms., Student’s t-test). ns, not significant. j Percent survival of the MKN-45 tumor-bearing mice. The rude mice were inoculated with subcutaneous MKN-45 tumors as described in (h), and the survival time was recorded. (n = 10, **P < 0.01, means ± s.e.ms., Gehan-Breslow-Wilcoxon test). ns, not significant

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