Fig. 3

NF-κB suppresses LPTS expression in the promoter level. a Schematic diagram of two constructs harboring the promoter regions (−1300/+25; −495/+25) of human LPTS gene. Two putative NF-κB binding elements locating at −1143/−1136 (tgggaaaa) and −888/−881 (tggagagt) were displayed. b Relative luciferase activity of CaSki cells transfected with pGL3-Basic, pGL3-LP(−495/+25) or pGL3-LP(−1300/+25) for 24 h.(n = 4, **P < 0.01, means ± s.e.ms., Student’s t-test.). c Schematic diagram of reporter constructs harboring the human LPTS promoter (−1300/+25) with different mutant elements for NF-κB as indicated. d Relative luciferase activity of CaSki cells transfected with pGL3-LP(−1300/+25), MUT-1, MUT-2 and MUT-3, respectively (n = 4, means ± s.e.ms., one-way ANOVA). Values not sharing a common superscript letter differ significantly. e The NF-κB binding elements were responsible for the activity of LPTS promoter. CaSki cells were transfected with pGL3-LP(−1300/+25) or MUT3, and then treated with BAY11–7082 (10 μM) for 24 h. Cells were harvested for luciferase activity assays. (n = 3, **P < 0.01, means ± s.e.ms., Student’s t-test.). f Relative luciferase activity of CaSki cells transfected with pGL3-LP(−1300/+25) or MUT3 plus with PLKO or P65-shRNA for 24 h. (n = 3, *P < 0.05, means ± s.e.ms., Student’s t-test)