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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: NF-κB potentiates tumor growth by suppressing a novel target LPTS

Fig. 2

NF-κB suppresses LPTS expression in mRNA and protein levels. a Relative NF-κB activity in CaSki cells. CaSki cells were transfected with a reporter gene containing consensus binding elements of NF-κB and treated with a NF-κB inhibitor BAY11–7082 (10 μM) for 24 h. Cells were harvested for luciferase activity assays. (n = 4, ***P < 0.005). b Relative mRNA levels of LPTS in CaSki cells treated with BAY11–7082 (10 μM) for 24 h. (n = 4, ***P < 0.005). c Immunoblotting assays of LPTS and P65 in CaSki cells as described in (b). Representative results were displayed. d Relative mRNA expression of LPTS in CaSki cells transfected with p65 shRNAs (shRNA-1 or shRNA-2) or PLKO vector as control. (n = 4, *P < 0.05 and **P < 0.01). e Relative NF-κB activity in CaSki cells transfected with p65 shRNAs or PLKO vector as control. (n = 4, *P < 0.05 and **P < 0.01). f Relative mRNA levels of LPTS in CaSki cells transfected with p65 shRNAs or PLKO vector as control. (n = 4, *P < 0.05 and **P < 0.01). g Immunoblotting assays of LPTS and P65 proteins in CaSki cells transfected with p65 shRNAs or PLKO vector as control. Representative results were displayed. All the histograms in this figure show the means ± s.e.ms., Student’s t-test

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